An assay to quantify satellite cell activation using isolated mouse myofibers.
Canibano-Fraile R, Boertjes E, Bozhilova S, Pijnappel WWMP, Schaaf GJ
STAR protocols, 2021 Jun 18
Abstract
Isolated myofibers offer the possibility of study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for experimentation.